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Index > Protein center > Igf2(Gene name) > Mouse
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  • Igf2 (Gene name),
  • Insulin-like growth factor II (Protein name ),  IGF2_MOUSE from NCBI database.
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  • General Annotation
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  • Antigen Annotation
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  • Predicted Eptitope
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  • Vaild Sequence
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    • Gene name:
    Igf2(Igf-2);
    Protein name:
    Insulin-like growth factor II;
    Alternative:
    Multiplication-stimulating polypeptide;IGF-II;
    Organism:
    Mouse (Mus musculus). 
    General Annotation
    Sub Unit:
    N/A
    Function:
    Preptin undergoes glucose-mediated co-secretion with insulin, and acts as physiological amplifier of glucose-mediated insulin secretion. Exhibits osteogenic properties by increasing osteoblast mitogenic activity through phosphoactivation of MAPK1 and MAPK3.
    Subcellular Location:
    Secreted
    Protein Attributes:
    Sequence length:
    180
    Sequence:
    50:
    MGIPVGKSML | VLLISLAFAL | CCIAAYGPGE | TLCGGELVDT | LQFVCSDRGF | 
    100:
    YFSRPSSRAN | RRSRGIVEEC | CFRSCDLALL | ETYCATPAKS | ERDVSTSQAV | 
    150:
    LPDDFPRYPV | GKFFQYDTWR | QSAGRLRRGL | PALLRARRGR | MLAKELKEFR | 
    180:
    EAKRHRPLIV | LPPKDPAHGG | ASSEMSSNHQ | 
    3D Structure:
    N/A
    Predicted Eptitope:
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    EIAab Sequence  Vaild Sequence:
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    Related Databases
    SMR:
    P09535
    KEGG:
    mmu:16002
    UniGene:
    Mm.3862
    String:
    P09535
    Pfam:
    PF08365
    Uniprot:
    P09535
     
    FOR
    ELISA Kit for Mouse Insulin-like growth factor II
    Cat.:
    E0051r
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    ELISA Kit for Mouse Insulin-like growth factor II
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    E0051p
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    ELISA Kit for Mouse Insulin-like growth factor II
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    E0051m
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    ELISA Kit for Mouse Insulin-like growth factor II
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    ELISA Kit for Mouse Insulin-like growth factor II
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    E0051c
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    ELISA Kit for Mouse Insulin-like growth factor II
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    E0051b
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    CLIA Kit for Mouse Insulin-like growth factor II
    Cat.:
    U0051b
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    CLIA Kit for Mouse Insulin-like growth factor II
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    U0051c
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    CLIA Kit for Mouse Insulin-like growth factor II
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    U0051h
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    CLIA Kit for Mouse Insulin-like growth factor II
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    CLIA Kit for Mouse Insulin-like growth factor II
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    CLIA Kit for Mouse Insulin-like growth factor II
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    Polyclonal Antibody for Mouse Insulin-like growth factor II
    Cat.:
    P0051Rb-m
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    Packing:
    40ug/0.2ml
    Polyclonal Antibody for Mouse Insulin-like growth factor II
    Polyclonal Antibody for Mouse Insulin-like growth factor II
    Cat.:
    P0051Rb-r
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    Packing:
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    Polyclonal Antibody for Mouse Insulin-like growth factor II
    Polyclonal Antibody for Mouse Insulin-like growth factor II
    Cat.:
    P0051Rb-h
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    Packing:
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    Polyclonal Antibody for Mouse Insulin-like growth factor II
    Monoclonal Antibody for Mouse Insulin-like growth factor II
    Monoclonal Antibody for Mouse Insulin-like growth factor II
    Monoclonal Antibody for Mouse Insulin-like growth factor II
    Monoclonal Antibody for Mouse Insulin-like growth factor II
    Monoclonal Antibody for Mouse Insulin-like growth factor II
    Monoclonal Antibody for Mouse Insulin-like growth factor II
    Protein for Mouse Insulin-like growth factor II
    Protein for Mouse Insulin-like growth factor II
    Protein for Mouse Insulin-like growth factor II
    Protein for Mouse Insulin-like growth factor II
    Protein for Mouse Insulin-like growth factor II
    Protein for Mouse Insulin-like growth factor II

    R&D Technical Data
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    s

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    Precision
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    Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

    Intra-Assay CV: ≤3.8%

    Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

    Inter-Assay CV: ≤6.7%

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    Recovery
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    Recovery was determined by spiking various levels of IGF2 into serum and plasma .

    Sample Type

    Average(%)

    Recovery Range(%)

    Serum

    91

    87-95

    Plasma

    92

    90-97

     

     

     

     

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    Linearity
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    The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IGF2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.  

     

    Sample

    1：2

    1:4

    1:8

    1:16

    serum(n=5)

    90-96%

    85-92%

    87-98%

    91-97%

    EDTA plasma(n=5)

    93-95%

    88-96%

    95-100%

    86-93%

    heparin plasma(n=5)

    88-94%

     

    91-98%

    100-106%

    80-87

     

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    References
    1. 1.
      "Sequence of a placental cDNA encoding the mouse insulin-like growth factor II precursor."
      Stempien M.M. , Fong N.M. , Rall L.B. , Bell G.I.
      DNA5:357-361(1986) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
    2. 2.
      "Evolution of insulin-like growth factor II: characterization of the mouse IGF-II gene and identification of two pseudo-exons."
      Rotwein P. , Hall L.J.
      DNA Cell Biol.9:725-735(1990) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]
    3. 3.
      "Nucleotide sequence of a 28-kb mouse genomic region comprising the imprinted Igf2 gene."
      Sasaki H. , Shimozaki K. , Zubair M. , Aoki N. , Hatano N. , Moore T. , Feil R. , Constancia M. , Reik W. , Rotwein P.
      DNA Res.3:331-335(1996) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]
    4. 4.
      "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res.14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]
      strain: C57BL/6J.
      tissue: Embryo.
    5. 5.
      "Coordinate expression of insulin-like growth factor II and its receptor during muscle differentiation."
      Tollefsen S.E. , Sadow J.L. , Rotwein P.
      Proc. Natl. Acad. Sci. U.S.A.86:1543-1547(1989) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-52
    6. 6.
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-52 AND 103-180
      strain: BALB/c.
      tissue: Spleen.
    7. 7.
      "Preptin derived from proinsulin-like growth factor II (proIGF-II) is secreted from pancreatic islet beta-cells and enhances insulin secretion."
      Buchanan C.M. , Phillips A.R. , Cooper G.J.
      Biochem. J.360:431-439(2001) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 93-126;INSULIN-ENHANCING FUNCTION OF PREPTIN
    8. 8.
      "Preptin, another peptide product of the pancreatic beta-cell, is osteogenic in vitro and in vivo."
      Cornish J. , Callon K.E. , Bava U. , Watson M. , Xu X. , Lin J.M. , Chan V.A. , Grey A.B. , Naot D. , Buchanan C.M. , Cooper G.J. , Reid I.R.
      Am. J. Physiol.292:E117-E122(2007) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: OSTEOGENIC FUNCTION OF PREPTIN
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